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KMID : 1024520080170091033
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Journal of the Environmental Sciences
2008 Volume.17 No. 9 p.1033 ~ p.1037
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Conditions for Soluble Phosphate Production by Environment-Friendly Biofertilizer Resources, Pseudomonas fluorescens
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Park Ki-Hyun
Park Geun-Tae
Kim Sung-Man
Lee Chung-Yeol
Son Hong-Joo
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Abstract
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The effects of inorganic salts, inoculum concentration, aeration rate and shaking speed on insoluble phosphate solubilization by Pseudomonas fluorescens RAF15 were investigated. Soluble phosphate production was dependent on the presence of and in the medium. Supplementation of medium with 0.01% and 0.01% NaCl slightly increased soluble phosphate production. The optimal medium compositions for the solubilization of insoluble phosphate by P. fluorescens RAF15 were 1.5% glucose, 0.005% urea, 0.3% , 0.01% , 0.01% and 0.01% NaCl, respectively. Optimal inoculum concentration was 2.0%(v/v). Maximum soluble phosphate production was obtained with 20-50 ml/250-ml flask and 200 rpm of shaking speed, respectively. The addition of EDTA decreased cell growth and soluble phosphate production.
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KEYWORD
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Biofertilizer, Phosphate-solubilizing bacteria, Pseudomonas fluorescens
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